Rapid Method for Isolation of Organic Poisons

Poisons: Their Isolation and Identification

Improved alkaline lysis method for rapid isolation of plasmid DNA.

Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...

A Rapid Differential Method for the Isolation of Bacillus Influenzæ

I t has often been noted that on a blood agar plate seeded with influenzal sputum the colonies of Bacillus influenz6e are most numerous and grow to larger size in the neighborhood of colonies of certain other organisms, notably the staphylococcus. This phenomenon led the authors to conduct various experiments which may be summarized as follows: Blood agar plates were seeded uniformly with a pur...

Rapid method for the isolation of mammalian sperm DNA.

The unique DNA packaging of spermatozoa renders them resistant to DNA isolation techniques used for somatic cells, requiring alternative methods that are slow and labor intensive. Here we present a rapid method for isolating high-quality sperm DNA. Isolated human sperm cells were homogenized with 0.2 mm steel beads for 5 min at room temperature in the presence of guanidine thiocyanate lysis buf...

A rapid method for isolation of double stranded RNA.

A rapid and simple method for the isolation and purification of dsRNA is presented. The crucial step of this method is the extraction of proteins and DNA with acid phenol. After the extraction, only RNA is left in the aqueous phase. ssRNA contamination of the RNA preparation can be greatly reduced when ammonium sulfate is present during the extraction.